Used in the isolation of: Neisseria meningitides 13090, Staphylococcus epidermidis 12228, Streptococcus pneumoniae 6305, Streptococcus pyogenes 19615, Aspergillus brasiliensis (niger) 16404, Bacillus subtillis 6633, Candida albicans 10231, Escherichia coli 8739, Pseudomonas aeruginosa 9027, Salmonella enterica subsp.Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products.TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi.
1 liter (35 imp fl oz 34 U.S.Tryptic Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.
A typical set of control organisms and growth associated with these is detailed in the chart below.
2.5 grams (0.088 oz) of dextrose ( glucose) Tryptic Soy Broth will support growth of a wide range of aerobic and anaerobic bacteria if incubated under the right conditions. 2.5 grams (0.088 oz) of dipotassium phosphate (K 2HPO 4) Trypticase soy broth ( BBL ) Trycase soy broth ( bioMrieux ) Bacto - tryptic soy broth ( Difco ) Tryptone soy ( GIBCO ) and Tryptone soy broth ( Oxoid ). 5 grams (0.18 oz) of Sodium Chloride ( NaCl). 3 grams (0.11 oz) of Phytone peptone (Soytone). 17 grams (0.60 oz) of Trypticase peptone ( Tryptone). The solution is then autoclaved for 15 minutes at 121 ☌ (250 ☏). Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25 ☌ (77 ☏). To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Dextrose is a carbohydrate that acts as a carbon, energy source. Please call us at 80 if youd like us to keep stock in the. Difco 211771 5 lb 402.76/EA Add to Cart This item is not currently stocked. The nutritive components include pancreatic digest of casein and papaic digest of soybean meal. Difco 211822 2 kg 335.56/EA Add to Cart 5 In Stock 5 In Stock. Tryptic Soy Broth MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes. Tryptic Soy Broth is a highly nutritious medium used for the cultivation of aerobes and facultative anaerobes, and some fungi. Casein-peptone soymeal-peptone broth for microbiology (According harm. One of the components of Tryptic soy broth is Phytone Archived at the Wayback Machine, which is an enzymatic digest of soybean meal. Its agar counterpart is tryptic soy agar (TSA). It is a complex, general purpose medium that is routinely used to grow certain pathogenic bacteria, which tend to have high nutritional requirements (i.e., they are fastidious). Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic bacteria. JSTOR ( April 2015) ( Learn how and when to remove this template message). 
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